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Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus BABT
Sanches,Márcio Martinello; Sihler,William; Silva,Cláudia Efigênia Pereira; Guimarães,Giovana Curcio; Benito,Norton Polo; Sosa-Gómez,Daniel Ricardo; de Souza,Marlinda Lobo.
Abstract Chrysodeixis includens has become the major Lepidopteran pest of soybean crops, especially in the Brazilian Cerrado (savanna) region. A native isolate of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) from this region, Buritis, MG, was assessed for its biological and molecular features. In addition, in vitro co-infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), another virus of an important soybean pest, was tested. The ChinNPV-Buritis isolate presented an average LC50 of 7,750 occlusion bodies (OBs)/ml of diet in C. includens larvae. Analysis of restriction endonuclease profiles of viral DNA revealed similarities with previously described ChinNPV isolates IE, IF, and IG from Brazil, although the presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AgMNPV; Baculovirus; ChinNPV; Soybean looper; Velvetbean caterpillar.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100218
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Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States PAB
Botelho,Stephanie Regina Alves; Duarte,Macária Ferreira; Barbosa,Andreza Viveiros; Lau,Douglas; Sanches,Márcio Martinello; Fernandes,Fernanda Rausch.
Abstract: The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real-time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays; Exotic pest; High plains virus; Plant quarantine; Phytosanitary analysis..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500688
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